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Medium composition for testing the effects of the antifibrotic molecules (AFM) caffeine, curcumin and pirfenidone in activated primary human corneal stromal keratocytes (aCSK).

Journal: International Journal of Molecular Sciences

Article Title: Antifibrotic Effects of Caffeine, Curcumin and Pirfenidone in Primary Human Keratocytes

doi: 10.3390/ijms24021461

Figure Lengend Snippet: Medium composition for testing the effects of the antifibrotic molecules (AFM) caffeine, curcumin and pirfenidone in activated primary human corneal stromal keratocytes (aCSK).

Article Snippet: basal medium = control , DMEM/Ham’s F12 (Merck) +1% MEM nonessential amino acids (Gibco) +0.8% Penicillin-Streptomycin (Sigma-Aldrich) +1% Amphotericin B (Sigma-Aldrich) +1% MEM Eagle’s Vitamin Mix (Merck) +1 mM L-ascorbate 2-phosphate (Sigma-Aldrich) +10 µM ROCK-inhibitor (AdooQ Bioscience) +10 ng/mL Insulin-like growth factor (Gibco) +0.5% FBS (Panbiotech).

Techniques: